Shotgun proteomics of extracellular matrix in late senescent human dermal fibroblasts reveals a down-regulated fibronectin-centered network

Abstract Extracellular matrix (ECM) proteins play a pivotal role in cell growth and differentiation. To characterize aged ECM proteins, we compared the proteomes by shotgun method of young (passage #15) late senescent #40) human dermal fibroblasts (HDFs) using SDS-PAGE coupled with LC–MS/MS. The relative abundance identified was determined mol% individual as semi-quantitative index. Fifteen including apolipoprotein B (APOB) high-temperature requirement factor 1 (HTRA1) were up-regulated, whereas 50 fibronectin (FN1) vitronectin (VTN) down-regulated HDFs. combined plasma membrane queried to construct protein–protein interaction network Ingenuity Pathways Analysis, resulting distinct FN1-centered network. Of differentially abundant proteomics, protein levels FN1, VTN, APOB, HTRA1 verified immunoblot analysis. results suggest that aging process HDFs might be finally involved impaired FN1 regulatory altered neighboring proteins. Shotgun proteomics highly provides insight for further studies senescence-related alterations